Identification and quantification of drugs, metabolites, drug metabolizing
| Other Authors: | , |
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| Language: | English |
| Published: |
Amsterdam :
Elsevier,
2020.
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| Subjects: | |
| Online Access: | ScienceDirect |
Table of Contents:
- Intro
- Identification and Quantification of Drugs, Metabolites, Drug Metabolizing Enzymes, and Transporters: Concepts, Methods, a ...
- Copyright
- Contents
- Contributors
- Foreword
- References
- Preface
- Part I: Techniques for identifying and quantifying drugs and metabolites
- Chapter 1: Bioanalysis of small and large molecule drugs, metabolites, and biomarkers by LC-MS
- 1. Introduction
- 2. Complexity of contemporary bioanalysis
- 3. Bioanalytical requirements for supporting discovery, nonclinical, and clinical studies
- 4. Current regulatory landscape for bioanalysis
- 5. General considerations for bioanalysis for sample collection
- 6. Diagnosis and mitigation of nonspecific adsorption loss for urine bioanalysis
- 7. Tissue bioanalysis
- 8. Managing unstable metabolites such as acyl glucuronide
- 9. General considerations for bioanalysis for extraction, chromatography, and MS detection
- 10. Selected applications for LC-MS bioanalysis
- 10.1. LC-MS of large molecules
- 10.1.1. LC-MS bioanalysis of mAb
- 10.1.2. LC-MS bioanalysis of ADC
- 10.1.3. LC-MS bioanalysis of PDC
- 10.1.4. LC-MS bioanalysis of protein biomarkers
- 10.3.3. Fatty acid amide hydrolase biomarkers (authentic matrix and surrogate analytes)
- 10.4. LC-MS analysis of metabolites and MIST
- 10.4.1. Metabolite quantitation strategy
- 10.4.2. LC-MS bioanalysis of polar metabolites
- 10.4.3. Bioanalysis of chiral compounds
- 10.4.4. Unexpected assay challenge during analysis of patient samples
- 11. Conclusion and future perspective
- References
- Chapter 2: Recent advances in mass spectrometric and other analytical techniques for the identification of drug metabolites
- 1. Introduction
- 2. Sample preparation strategies
- 2.1. Solid-phase extraction
- 2.2. Supported phase liquid extraction
- 2.3. Liquid-liquid extraction
- 2.4. Protein precipitation
- 3. Optical detectors and chromatographic separation techniques
- 3.1. Ultra-performance liquid chromatography
- 3.2. Supercritical fluid chromatography
- 4. Different types of Ionization techniques and mass spectrometric scan functions
- 4.1. Ionization techniques
- 4.2. Mass analyzers and different acquisition modes
- 4.3. Inductively coupled mass spectrometry (ICP-MS)
- 5. Wet chemistry techniques combined with MS