Immunology: Overview and Laboratory Manual
A two-in-one text providing teaching lab students with an overview of immunology as well as a lab manual complete with current standard exercises. Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based q...
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| Language: | English |
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Cham :
Springer International Publishing : Imprint: Springer,
2021.
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| Edition: | 1st ed. 2021. |
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| Online Access: | https://doi.org/10.1007/978-3-030-64686-8 |
Table of Contents:
- Part I: Overview of Immunity
- Ch 1: Immunity
- Ch 2: Components of immunity
- Ch 3: Hematopoiesis
- Ch 4: The Immune system: pathogen sensing and detection
- Ch 5: Lymphoid organs
- Ch 6: Innate and Adaptive immunity
- Ch 7: Leukocyte homing, migration and recirculation
- Ch 8: Apoptosis
- Ch 9: Antigens
- Ch 10: B cell development, activation and immunoglobulin structure
- Ch 11: Immunoglobulin and T cell receptor gene rearrangements
- Ch 12: Major histocompatibility complex proteins
- Ch 13: T cell development and T cell receptor structure
- Ch 14: Cytokines
- Ch 15: Lymphocyte signals
- Ch 16: Complement fixation
- Ch 17: Cell mediated immunity
- Ch 18: Hypersensitivities
- Ch 19: Parasite Immunity
- Ch 20: Vaccines
- Ch 21: Transplantation
- Ch 22: Immune regulation and autoimmunity
- Ch 23: Immunodeficiencies
- Ch 24: Cancer
- Ch 25: Practice questions
- Ch 26: Study guide for exam. Part II. Laboratory Manual
- 27. Immunology laboratory policies
- 28. Instructions for notebook entries
- 29. Instructions for writing lab reports
- 30. Lab report topics
- 31. Exercise 1: Using a compound microscope
- 32. Exercise 2: Pipetting and dilution technique, identification of mouse lymphoid organs
- 33. Exercise 3: Blood Cell Preparation, Leukocyte differentiation and ABO Blood Typing
- 34. Exercise 4: Single Suspension of Mouse Spleen cells, Cell Viability Assays, and Identification of specific cells using surface antigens
- 35. Exercise 5: Isolation of mouse peritoneal macrophages: In vitro phagocytosis of bacterial cells
- 36. Exercise 6: Clearance of bacteria from mouse blood by the RES
- 37. Exercise 7: Hemolytic Plaque assay (The Jeme Plaque Assay)
- 38. EXERCISE 8: Rabbit and Mouse Immunizations- Preparation of Polyclonal antibodies and screening assays
- 39. EXERCISE 9: Determination of Antibody Titer: Screening Techniques
- 40. EXERCISE 10: Immunodiffusion and Immunoelectrophoresis
- 41. EXERCISE 11: Protein Affinity Purification of Rabbit IgG
- 42. EXERCISE 12: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
- 43. EXERCISE 13: Immunoblotting (Western Blotting)
- 44. Exercise 14: Protein Assay
- 45. Exercise 15: Enzyme-linked Immunosorbent Assay (ELISA)
- 46. EXERCISE 16: Enzyme-Linked Immunospot Assay (ELISPOT)
- 47. EXERCISE 17: Immunofluorescence assay (IFA)
- 48. EXERCISE 18: Flow Cytometry
- 49.EXERCISE 19: Complement Fixation Assays
- 50. Tissue Cell Culture Techniques
- 51. EXERCISE 20: Mitogen Induced Response of Lymphocytes: Detection of Band T cell Lymphocytes Using Immunofluorescence
- 52. Exercise 21: Induction of IL-2 Secretion from Mouse Lymphoma Cells
- 53. EXERCISE 22: Adoptive Transfer of Lymphocytes: Band T cell Cooperation for Antibody Production
- 54. EXERCISE 23: Macrophage Migration Inhibition
- 55. EXERCISE 24: Monoclonal Antibody Preparation
- 56. EXERCISE 25: Immunization of mice with a recombinant protein
- 57. EXERCISE 26: Polarization of T helper cells into TH1 subset
- 58. Exercise 27: Immunoprecipitation
- 59. EXERCISE 28: Induction of apoptosis in T lymphocytes and detection of apoptotic markers
- 60. EXERCISE 29: Cloning and sequencing of cDNAs of mouse variable regions of H and L chains of monoclonal antibodies
- Appendix
- Sample Practice Questions.