|
|
|
|
| LEADER |
05888nam a22004695i 4500 |
| 001 |
978-3-030-64686-8 |
| 003 |
DE-He213 |
| 005 |
20210801145047.0 |
| 007 |
cr nn 008mamaa |
| 008 |
210801s2021 gw | s |||| 0|eng d |
| 020 |
|
|
|a 9783030646868
|9 978-3-030-64686-8
|
| 024 |
7 |
|
|a 10.1007/978-3-030-64686-8
|2 doi
|
| 050 |
|
4 |
|a QR180-189.5
|
| 072 |
|
7 |
|a MJCM
|2 bicssc
|
| 072 |
|
7 |
|a MED044000
|2 bisacsh
|
| 072 |
|
7 |
|a MJCM
|2 thema
|
| 082 |
0 |
4 |
|a 616.079
|2 23
|
| 100 |
1 |
|
|a Sam-Yellowe, Tobili Y.
|e author.
|4 aut
|4 http://id.loc.gov/vocabulary/relators/aut
|
| 245 |
1 |
0 |
|a Immunology: Overview and Laboratory Manual
|h [electronic resource] /
|c by Tobili Y. Sam-Yellowe.
|
| 250 |
|
|
|a 1st ed. 2021.
|
| 264 |
|
1 |
|a Cham :
|b Springer International Publishing :
|b Imprint: Springer,
|c 2021.
|
| 300 |
|
|
|a XX, 471 p. 61 illus., 26 illus. in color.
|b online resource.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 347 |
|
|
|a text file
|b PDF
|2 rda
|
| 505 |
0 |
|
|a Part I: Overview of Immunity -- Ch 1: Immunity -- Ch 2: Components of immunity -- Ch 3: Hematopoiesis -- Ch 4: The Immune system: pathogen sensing and detection -- Ch 5: Lymphoid organs -- Ch 6: Innate and Adaptive immunity -- Ch 7: Leukocyte homing, migration and recirculation -- Ch 8: Apoptosis -- Ch 9: Antigens -- Ch 10: B cell development, activation and immunoglobulin structure -- Ch 11: Immunoglobulin and T cell receptor gene rearrangements -- Ch 12: Major histocompatibility complex proteins -- Ch 13: T cell development and T cell receptor structure -- Ch 14: Cytokines -- Ch 15: Lymphocyte signals -- Ch 16: Complement fixation -- Ch 17: Cell mediated immunity -- Ch 18: Hypersensitivities -- Ch 19: Parasite Immunity -- Ch 20: Vaccines -- Ch 21: Transplantation -- Ch 22: Immune regulation and autoimmunity -- Ch 23: Immunodeficiencies -- Ch 24: Cancer -- Ch 25: Practice questions -- Ch 26: Study guide for exam. Part II. Laboratory Manual -- 27. Immunology laboratory policies -- 28. Instructions for notebook entries -- 29. Instructions for writing lab reports -- 30. Lab report topics -- 31. Exercise 1: Using a compound microscope -- 32. Exercise 2: Pipetting and dilution technique, identification of mouse lymphoid organs -- 33. Exercise 3: Blood Cell Preparation, Leukocyte differentiation and ABO Blood Typing -- 34. Exercise 4: Single Suspension of Mouse Spleen cells, Cell Viability Assays, and Identification of specific cells using surface antigens -- 35. Exercise 5: Isolation of mouse peritoneal macrophages: In vitro phagocytosis of bacterial cells -- 36. Exercise 6: Clearance of bacteria from mouse blood by the RES -- 37. Exercise 7: Hemolytic Plaque assay (The Jeme Plaque Assay) -- 38. EXERCISE 8: Rabbit and Mouse Immunizations- Preparation of Polyclonal antibodies and screening assays -- 39. EXERCISE 9: Determination of Antibody Titer: Screening Techniques -- 40. EXERCISE 10: Immunodiffusion and Immunoelectrophoresis -- 41. EXERCISE 11: Protein Affinity Purification of Rabbit IgG -- 42. EXERCISE 12: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) -- 43. EXERCISE 13: Immunoblotting (Western Blotting) -- 44. Exercise 14: Protein Assay -- 45. Exercise 15: Enzyme-linked Immunosorbent Assay (ELISA) -- 46. EXERCISE 16: Enzyme-Linked Immunospot Assay (ELISPOT) -- 47. EXERCISE 17: Immunofluorescence assay (IFA) -- 48. EXERCISE 18: Flow Cytometry -- 49.EXERCISE 19: Complement Fixation Assays -- 50. Tissue Cell Culture Techniques -- 51. EXERCISE 20: Mitogen Induced Response of Lymphocytes: Detection of Band T cell Lymphocytes Using Immunofluorescence -- 52. Exercise 21: Induction of IL-2 Secretion from Mouse Lymphoma Cells -- 53. EXERCISE 22: Adoptive Transfer of Lymphocytes: Band T cell Cooperation for Antibody Production -- 54. EXERCISE 23: Macrophage Migration Inhibition -- 55. EXERCISE 24: Monoclonal Antibody Preparation -- 56. EXERCISE 25: Immunization of mice with a recombinant protein -- 57. EXERCISE 26: Polarization of T helper cells into TH1 subset -- 58. Exercise 27: Immunoprecipitation -- 59. EXERCISE 28: Induction of apoptosis in T lymphocytes and detection of apoptotic markers -- 60. EXERCISE 29: Cloning and sequencing of cDNAs of mouse variable regions of H and L chains of monoclonal antibodies -- Appendix -- Sample Practice Questions.
|
| 520 |
|
|
|a A two-in-one text providing teaching lab students with an overview of immunology as well as a lab manual complete with current standard exercises. Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based questions, and more to provide students with a strong foundation in the field. Section II consists of twenty-two lab exercises focused on key concepts in immunology, such as antibody production, cell separation, cell function, immunoassays, Th1/Th2 cytokine detection, cell and tissue culture methods, and cell and molecular biology techniques. Appendices include safety information, suggested links and readings, and standard discipline processes, protocols, and instructions.
|
| 650 |
|
0 |
|a Immunology.
|
| 650 |
|
0 |
|a Laboratory medicine.
|
| 650 |
1 |
4 |
|a Immunology.
|0 https://scigraph.springernature.com/ontologies/product-market-codes/B14000
|
| 650 |
2 |
4 |
|a Laboratory Medicine.
|0 https://scigraph.springernature.com/ontologies/product-market-codes/B15007
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer Nature eBook
|
| 776 |
0 |
8 |
|i Printed edition:
|z 9783030646851
|
| 776 |
0 |
8 |
|i Printed edition:
|z 9783030646875
|
| 776 |
0 |
8 |
|i Printed edition:
|z 9783030646882
|
| 856 |
4 |
0 |
|u https://doi.org/10.1007/978-3-030-64686-8
|
| 912 |
|
|
|a ZDB-2-SBL
|
| 912 |
|
|
|a ZDB-2-SXB
|
| 950 |
|
|
|a Biomedical and Life Sciences (SpringerNature-11642)
|
| 950 |
|
|
|a Biomedical and Life Sciences (R0) (SpringerNature-43708)
|
