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Flow Cytometry Basics for the Non-Expert

Flow cytometry allows for the measurement of different aspects of a cell or particles in real time. Applications include quantifying various types of T cells to determine the presence of various immune disorders, such as whether HIV has progressed to AIDS, studying the dynamics of immune signaling i...

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Main Authors: Goetz, Christine. (Author, http://id.loc.gov/vocabulary/relators/aut), Hammerbeck, Christopher. (http://id.loc.gov/vocabulary/relators/aut), Bonnevier, Jody. (http://id.loc.gov/vocabulary/relators/aut)
Corporate Author: SpringerLink (Online service)
Language:English
Published: Cham : Springer International Publishing : Imprint: Springer, 2018.
Edition:1st ed. 2018.
Series:Techniques in Life Science and Biomedicine for the Non-Expert,
Subjects:
Antibodies.
Laboratory medicine.
Laboratory Medicine.
Online Access:https://doi.org/10.1007/978-3-319-98071-3
Table of Contents:
  • 1. Flow Cytometry: Definition, History, and Uses in Biotechnology
  • 2. Physics of a Flow Cytometer
  • 3. The Language of Flow Cytometry and Experimental Setup
  • 4. Fluorochrome Choices for Flow Cytometry
  • 5. Compensation
  • 6. Primary and Seconardy Antibodies and Their Use in Flow Cytometry
  • 7. Experimental Considerations with Data Sets as Examples
  • 8. Cell Enrichment
  • 9. Surface and Intracellular Staining Protocols for Flow Cytometry
  • 10. Troubleshooting.

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