Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing
High throughput sequencing (HTS) technologies have conquered the genomics and epigenomics worlds. The applications of HTS methods are wide, and can be used to sequence everything from whole or partial genomes, transcriptomes, non-coding RNAs, ribosome profiling, to single-cell sequencing. Having suc...
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| Other Authors: | , |
| Language: | English |
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Cham :
Springer International Publishing : Imprint: Springer,
2016.
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| Edition: | 1st ed. 2016. |
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| Online Access: | https://doi.org/10.1007/978-3-319-31350-4 |
Table of Contents:
- The High Throughput Sequencing technologies triple-W discussion: Why to use HTS, What is the optimal HTS method to use, Which data analysis workflow to follow
- Whole genome sequencing recommendations
- Counseling for targeted DNA region re-sequencing
- Transcriptome profiling strategies
- Differential mRNA Alternative Splicing
- microRNA discovery and expression analysis in animals
- Analysis of Long Non-coding RNAs in RNA-seq data
- Ribosome profiling
- Genome-wide analysis of DNA methylation patterns by high-throughput sequencing
- Characterization of DNA-protein interactions: Design and analysis of ChIP-seq experiments
- PAR-CLIP: A genomic technique to dissect RNA-protein interactions
- Metagenomic design and sequencing
- A hitchhiker's guide to Metatranscriptomics
- Eukaryotic Single-Cell mRNA Sequencing
- Eukaryotic Single-Cell DNA Sequencing
- Submitting data to a public repository, the final step of a successful HTS experiment.