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Low-Cost Methods for Molecular Characterization of Mutant Plants Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols /

This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single...

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Main Authors: Till, Bradley J. (Author, http://id.loc.gov/vocabulary/relators/aut), Jankowicz-Cieslak, Joanna. (http://id.loc.gov/vocabulary/relators/aut), Huynh, Owen A. (http://id.loc.gov/vocabulary/relators/aut), Beshir, Mayada M. (http://id.loc.gov/vocabulary/relators/aut), Laport, Robert G. (http://id.loc.gov/vocabulary/relators/aut), Hofinger, Bernhard J. (http://id.loc.gov/vocabulary/relators/aut)
Corporate Author: SpringerLink (Online service)
Language:English
Published: Cham : Springer International Publishing : Imprint: Springer, 2015.
Edition:1st ed. 2015.
Subjects:
Plant breeding.
Biology—Technique.
Nucleic acids.
Plant Breeding/Biotechnology.
Biological Techniques.
Nucleic Acid Chemistry.
Online Access:https://doi.org/10.1007/978-3-319-16259-1
Table of Contents:
  • Introduction
  • Health and Safety Considerations
  • Sample Collection and Storage
  • Low-Cost DNA Extraction
  • PCR Amplification for Low-Cost Mutation Discovery
  • Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples
  • Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants
  • Example Data
  • Conclusions.  .

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