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Detection and Typing Strategies for Pathogenic Escherichia coli

This Brief will review the methods that are currently available for the detection, isolation, and typing of pathogenic E. coli with a particular focus on foodborne diseases caused by the Shiga toxigenic E. coli group, which have been implicated in a number of significant outbreaks in recent years. P...

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Main Authors: Rivas, Lucia. (Author, http://id.loc.gov/vocabulary/relators/aut), Mellor, Glen E. (http://id.loc.gov/vocabulary/relators/aut), Gobius, Kari. (http://id.loc.gov/vocabulary/relators/aut), Fegan, Narelle. (http://id.loc.gov/vocabulary/relators/aut)
Corporate Author: SpringerLink (Online service)
Language:English
Published: New York, NY : Springer New York : Imprint: Springer, 2015.
Edition:1st ed. 2015.
Series:SpringerBriefs in Food, Health, and Nutrition,
Subjects:
Food—Biotechnology.
Bacteriology.
Microbiology.
Food Science.
Applied Microbiology.
Online Access:https://doi.org/10.1007/978-1-4939-2346-5
Table of Contents:
  • Chapter 1 Introduction to Pathogenic Escherichia coli
  • 1.1 Pathotypes of Escherichia coli causing diarrhoeal diseases
  • 1.2 Escherichia coli
  • 1.3 Pathogenic types of E. coli
  • 1.4 Shiga toxin-producing and Enterohemorrhagic E. coli
  • 1.4.1    Virulence determinants of Shiga-toxin producing E. coli
  • 1.4.2 Shiga-toxin producing E. coli-mediated disease
  • 1.4.3 Epidemiology of Shiga toxin-producing E. coli
  • 1.4.4    Ecology of Shiga-toxin producing E. coli
  • 1.4.5 Transmission of Shiga-toxin producing E. coli
  • 1.4.6 Shiga-toxin producing E. coli occurrence in foods
  • 1.5 Enterotoxigenic E. coli
  • 1.6 Enteropathogenic E. coli
  • 1.7 Enteroinvasive E. coli
  • 1.8 Enteroaggregative and Diffusely Adherent E. coli..-1.9 References
  • Chapter 2 Isolation and detection of pathogenic Escherichia coli in foods
  • 2.1 Introduction
  • 2.2 General method of isolation for E. coli
  • 2.3 Shiga toxin-producing E. coli
  • 2.3.1 Culture and isolation of Shiga toxin-producing E. coli
  • 2.3.2    Molecular detection of Shiga toxin-producing E. coli
  • 2.4 Enumeration of Shiga toxin-producing E.coli
  • 2.5 Immunological detection methods for pathogenic E. coli
  • 2.6 Cell culture assays used for pathogenic E. coli
  • 2.6.1 Cell cytotoxicity assay for Shiga toxin-producing E. coli
  • 2.6.2    Cell adherence assays
  • 2.7 Enteroinvasive E. coli
  • 2.8 Enterotoxigenic E. coli
  • 2.9 Enteropathogenic E. coli
  • 2.10     Enteroaggregative E. coli and Diffusely Adherent E. coli
  • 2.11 References
  • Chapter 3 Typing and Subtyping methods for pathogenic Escherichia coli
  • 3.1 Introduction
  • 3.2 Biochemical profiling
  • 3.3 Serotyping
  • 3.4 Phage typing
  • 3.5 Multilocus enzyme electrophoresis
  • 3.6 Multilocus sequence typing
  • 3.7 Pulsed-field gel electrophoresis
  • 3.8 Multiple-locus variable number tandem repeat analysis
  • 3.9 Repetitive element palindromic polymerase chain reaction
  • 3.10 Random amplified polymorphic DNA
  • 3.11 Shiga toxin subtypes and bacteriophage insertion sites
  • 3.12 Lineage specific polymorphism analysis
  • 3.13 Whole genome sequencing
  • 3.14
  • References
  • Chapter 4 Emerging and future trends and technologies for the detection and typing of Escherichia coli
  • 4.1 Future trends in foodborne illness and E. coli
  • 4.2 Future trends in the detection and typing of pathogens
  • 4.2.1 Microarrays
  • 4.2.2 Matrix Assisted Laser-Desorption Ionization-Time-Of-Flight Mass Spectrometry
  • 4.2.3 Nanotechnology
  • 4.2.4 Next-generating sequencing of the whole bacterial genome
  • 4.3 References.

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