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| LEADER |
01233cam a2200205 7i4500 |
| 001 |
0000026370 |
| 005 |
20111018090000.0 |
| 008 |
111018s1996 v eng |
| 090 |
0 |
0 |
|a QP 606 .D46
|b D5 1996
|
| 245 |
0 |
0 |
|a DNA sequencing using PCR.
|
| 260 |
# |
# |
|a Logan, UT:
|b Taped Technologies,
|c 1996.
|
| 300 |
# |
# |
|a 1 videocassette (30 min.):
|b sd., col.;
|c 7 1/2in..
|
| 490 |
0 |
0 |
|a Videos for science
|
| 520 |
0 |
# |
|a In the video, the plus and minus strands of PCR-amplified DNA were separated from each other. Magnetic beads coated with streptavidin were used to capture one of the PCR strands which had been labeled with biotin during the PCR. The strands were separated from each other by washing one strand away from the other strand which was attached to the magnetic beads. These strands served as single-stranded templates for the labeling and termination sequencing reactions. Electrophoresis of the reaction products and interpretation of the results were outlined.
|
| 538 |
0 |
0 |
|a VHS
|
| 650 |
0 |
0 |
|a DNA polymerases
|
| 650 |
0 |
0 |
|a Gene amplification --
|x Methodology
|
| 650 |
0 |
0 |
|a Polymerase chain reaction --
|x Methodology
|
| 710 |
2 |
1 |
|a Taped Technologies
|
| 999 |
|
|
|a 1100014423
|b Video
|c BAHAN SUMBER MEDIA
|e BILIK BAHAN SUMBER MEDIA
|