05303nam a22005775i 4500 978-3-319-10142-2 DE-He213 20210619194058.0 cr nn 008mamaa 141028s2015 gw | s |||| 0|eng d 9783319101422 978-3-319-10142-2 10.1007/978-3-319-10142-2 doi QH491 PSC bicssc SCI072000 bisacsh PSC thema 571.8 23 Genetic Transformation Systems in Fungi, Volume 1 [electronic resource] / edited by Marco A. van den Berg, Karunakaran Maruthachalam. 1st ed. 2015. Cham : Springer International Publishing : Imprint: Springer, 2015. XV, 318 p. 69 illus., 17 illus. in color. online resource. text txt rdacontent computer c rdamedia online resource cr rdacarrier text file PDF rda Fungal Biology, 2198-7777 Fungal Transformation: From Protoplasts to Targeted Recombination Systems -- Protoplast Transformation for Genome Manipulation in Fungi -- Trichoderma Transformation Methods -- Transformation of Mucor circinelloides f. lusitanicus Protoplasts -- Transformation of Saccharomyces cerevisiae: Spheroplast Method -- Electroporation Mediated DNA Transformation of Filamentous Fungi -- Chemical Transformation of Candida albicans -- Electroporation of Pichia pastoris -- Insertional Mutagenesis of the Flavinogenic Yeast Candida famata (Candida flareli) -- Biolistic Transformation for Delivering DNA into the Mitochondria -- Bolistic Transformation of Candida glabrata for Homoplasmic Mitochondrial Genome Transformants -- Use of the Biolistic Particle Delivery System to Transform Fungal Genomes -- Transformation of Zygomycete Mortierella alpina Using Biolistic Particle Bombardment -- Agrobacterium tumefaciens-Mediated Transformation -- Agrobacterium tumefaciens-Mediated Transformation of Pucciniomycotina Red Yeasts -- Glass-bead and Agrobacterium-mediated Genetic Transformation of Fusarium oxysporum -- High Efficiency DNA Transformation of Saccharomyces cerevisiae with the LiAc/SS-DNA/PEG Method -- Transformation of Intact Cells of Saccharomyces cerevisiae: Lithium Methods and Possible Underlying Mechanism -- Transformation of Lithium Acetate-treated Neurospora crassa -- Application of Novel Polymeric Carrier of Plasmid DNA for Transformation of Yeast Cells -- Transformation of Fungi Using Shock Waves -- Pathways and Mechanisms of Yeast Competence: A New Frontier of Yeast Genetics -- Evaluation of Competence Phenomenon of Yeast Saccharomyces cerevisiae by Lipophilic Cations Accumulation and FT-IR Spectroscopy. Relation of Competence to Cell Cycle -- Recombination and Gene Targeting in Neurospora -- Efficient Generation of Aspergillus niger Knock Out strains by Combining NHEJ Mutants and a Split Marker Approach -- REMI in Molecular Fungal Biology -- TALEN-Based Genome Editing in Yeast. Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples used in laboratories worldwide. Not only will this text provide a detailed background on applications in industrial and pharmaceutical relevant microbes, but also the importance of fungal pathogens in agricultural production (Phytophthora and Botrytis) and mammalian infection (Penicillium marneffei and Candida). Genetic Transformation Systems in Fungi, Volume 1 provides in-depth coverage of how the transformation of DNA is used to understand the genetic basis behind these fungal traits. Developmental biology. Plant genetics. Plant pathology. Plant breeding. Systems biology. Developmental Biology. https://scigraph.springernature.com/ontologies/product-market-codes/L18000 Plant Genetics and Genomics. https://scigraph.springernature.com/ontologies/product-market-codes/L32020 Plant Pathology. https://scigraph.springernature.com/ontologies/product-market-codes/L24035 Plant Breeding/Biotechnology. https://scigraph.springernature.com/ontologies/product-market-codes/L24060 Systems Biology. https://scigraph.springernature.com/ontologies/product-market-codes/L15010 van den Berg, Marco A. editor. edt http://id.loc.gov/vocabulary/relators/edt Maruthachalam, Karunakaran. editor. edt http://id.loc.gov/vocabulary/relators/edt SpringerLink (Online service) Springer Nature eBook Printed edition: 9783319101439 Printed edition: 9783319101415 Printed edition: 9783319379708 Fungal Biology, 2198-7777 https://doi.org/10.1007/978-3-319-10142-2 ZDB-2-SBL ZDB-2-SXB Biomedical and Life Sciences (SpringerNature-11642) Biomedical and Life Sciences (R0) (SpringerNature-43708)